VedDesign - Catalogues - flow cytometry data

Catalogues

:: Presentation is key in conferring the potential value of a reagent; a clear well thought-out figure reflects positively on the product, which in turn can facilitate experimentally superior results. The need for concise flow cytometry profiles is essential in promoting and demonstrating the quality of each product.

:: VedDesign offers unique expertise through the collaboration of senior scientists and design artists. Flow cytometry presentation is essential in promoting each product. The quality of each display is dependant on the question being posed, as well as its frequency in experimental systems and potential applications.

The Four Principal Flow Cytometry Displays

The four principal types of flow cytometry display are shown below; each of the markers depicted occur at low-frequency in vivo in humans (CD8+ T lymphocytes expressing the MHC class II antigen). The adjacent profiles demonstrate the value of utilizing the accumulated expertise of VedDesign in analyzing and presenting flow cytometry plots.

Dotplot	Before	VedDesign	Info
			The dot-plots depict HLA-DR expression on CD8+ T lymphocytes. These types of profiles are of particular use in the resolution of cells that occur at low frequency, or have markers which are either weakly expressed or are present at low levels. The left profile shows an unclear poorly resolved profile, commonly shown in many flow cytometry catalogs. A clearer better defined dot-plot is shown in the right column with a lower level of autofluorescence and non-specific binding which is a result of inaccurate gating.
Densityplot	Before	VedDesign	Info
			Expression of HLA-DR on CD8+ T cells is shown by density-plots. These profiles are good for gross analysis, but lack resolution or the ability to reliably discriminate between positive and negative cells. The adjacent density-plots demonstrate staining and the presence of HLA-DR+ CD8+ T lymphocytes- but not clearly. However, the right panel shows the double-positive in the upper-right quadrant providing greater confidence in the assay as well as the reagents used.
Contourplot	Before	VedDesign	Info
			Use of contour plots is widespread in flow cytometry presentations, of which examples are shown in the adjacent panels. This method of analysis and presentation of data to scientists with flow cytometry understanding in academic, pharmaceutical and commercial environments suggests the reagent of interest is poor in producing a clean population of cells. The analysis shown in the right panel is a higher resolution contour plot used to clearly identify CD8+ HLA-DR+ cells present in the upper right quadrant.
Histogram	Before	VedDesign	Info
			The use of histograms in flow cytometry presentation is common place. They produce a very clean and understandable profile when using logical gating to identify cells occurring at low frequency or weakly expressed cell surface markers. However, histograms with contaminating cells or low cell counts produce a confusing picture, which is shown in the left panel. This is a typically observed histogram in many commercial catalogs, which does not inspire confidence in the reagent or its use in experimental assays. Both histograms show HLA-DR+ cells on CD8+ T cells , and are created from the same data file. The right panel by VedDesign clearly demonstrates the importance of concise flow cytometry analysis and presentation when promoting reagents.

Dotplot

Before

VedDesign

Info

The dot-plots depict HLA-DR expression on CD8+ T lymphocytes. These types of profiles are of particular use in the resolution of cells that occur at low frequency, or have markers which are either weakly expressed or are present at low levels. The left profile shows an unclear poorly resolved profile, commonly shown in many flow cytometry catalogs. A clearer better defined dot-plot is shown in the right column with a lower level of autofluorescence and non-specific binding which is a result of inaccurate gating.

Densityplot

Before

VedDesign

Info

Expression of HLA-DR on CD8+ T cells is shown by density-plots. These profiles are good for gross analysis, but lack resolution or the ability to reliably discriminate between positive and negative cells. The adjacent density-plots demonstrate staining and the presence of HLA-DR+ CD8+ T lymphocytes- but not clearly. However, the right panel shows the double-positive in the upper-right quadrant providing greater confidence in the assay as well as the reagents used.

Contourplot

Before

VedDesign

Info

Use of contour plots is widespread in flow cytometry presentations, of which examples are shown in the adjacent panels. This method of analysis and presentation of data to scientists with flow cytometry understanding in academic, pharmaceutical and commercial environments suggests the reagent of interest is poor in producing a clean population of cells. The analysis shown in the right panel is a higher resolution contour plot used to clearly identify CD8+ HLA-DR+ cells present in the upper right quadrant.

Histogram

Before

VedDesign

Info

The use of histograms in flow cytometry presentation is common place. They produce a very clean and understandable profile when using logical gating to identify cells occurring at low frequency or weakly expressed cell surface markers. However, histograms with contaminating cells or low cell counts produce a confusing picture, which is shown in the left panel. This is a typically observed histogram in many commercial catalogs, which does not inspire confidence in the reagent or its use in experimental assays. Both histograms show HLA-DR+ cells on CD8+ T cells , and are created from the same data file. The right panel by VedDesign clearly demonstrates the importance of concise flow cytometry analysis and presentation when promoting reagents.